Dietary Nitrate Supplementation Enhances Muscle

Dietary Nitrate Supplementation Enhances Muscle

Dietary nitrate supplementation enhances muscle contractile efficiency during knee-extensor exercise in humans

The purpose of this study was to elucidate the mechanistic bases for the reported reduction in the O2 cost of exercise following short-term dietary nitrate (NO3-) supplementation. In a randomized, double-blind, crossover study, seven men (aged 19-38 yr) consumed 500 ml/day of either nitrate-rich beetroot juice (BR, 5.1 mmol of NO3-/day) or placebo (PL, with negligible nitrate content) for 6 consecutive days, and completed a series of low-intensity and high-intensity "step" exercise tests on the last 3 days for the determination of the muscle metabolic (using 31P-MRS) and pulmonary oxygen uptake (Vo2) responses to exercise. On days 4-6, BR resulted in a significant increase in plasma [nitrite] (mean ± SE, PL 231 ± 76 vs. BR 547 ± 55 nM; P < 0.05). During low-intensity exercise, BR attenuated the reduction in muscle phosphocreatine concentration ([PCr]; PL 8.1 ± 1.2 vs. BR 5.2 ± 0.8 mM; P < 0.05) and the increase in Vo2 (PL 484 ± 41 vs. BR 362 ± 30 ml/min; P < 0.05). During high-intensity exercise, BR reduced the amplitudes of the [PCr] (PL 3.9 ± 1.1 vs. BR 1.6 ± 0.7 mM; P < 0.05) and Vo2 (PL 209 ± 30 vs. BR 100 ± 26 ml/min; P < 0.05) slow components and improved time to exhaustion (PL 586 ± 80 vs. BR 734 ± 109 s; P < 0.01). The total ATP turnover rate was estimated to be less for both low-intensity (PL 296 ± 58 vs. BR 192 ± 38 µM/s; P < 0.05) and high-intensity (PL 607 ± 65 vs. BR 436 ± 43 µM/s; P < 0.05) exercise. Thus the reduced O2 cost of exercise following dietary NO3- supplementation appears to be due to a reduced ATP cost of muscle force production. The reduced muscle metabolic perturbation with NO3- supplementation allowed high-intensity exercise to be tolerated for a greater period of time. Ref. Source 1